摘要
A cDNA library was prepared from mRNA extracted from bovine pituitaries,β-subunitDNA of bFSH was amplified from cDNA using Polymerase Chain Reaction(PCR).The sequence of aminoacid encoded by cDNA was the same as that of natural bFSH by sequencing.Signal peptide DNAfragment of bFSH was deleted by PCR mediated mutagenesis,to which PGEX-2T was ligated,yielding a high expression fusion protein of 40 kD.A recombinant bFSH fusion protein was extracted usingthrombin,yielding a pure bFSH of 14kD,showing positive reaction using Western blot analysis,and someactivation by biological tests with the mouse and the rabbit.
A cDNA library was prepared from mRNA extracted from bovine pituitaries,β-subunit DNA of bFSH was amplified from cDNA using Polymerase Chain Reaction(PCR).The sequence of amino acid encoded by cDNA was the same as that of natural bFSH by sequencing.Signal peptide DNA fragment of bFSH was deleted by PCR mediated mutagenesis,to which PGEX-2T was ligated, yielding a high expression fusion protein of 40 kD.A recombinant bFSH fusion protein was extracted using thrombin,yielding a pure bFSH of 14kD,showing positive reaction using Western blot analysis,and some activation by biological tests with the mouse and the rabbit.
