摘要
The levels of DNA polymerase α,δ,ε were examined in the neonatal mouse forebrains andspleens.The levels of DNA polymerase α were determined by the difference of polymerase activity in theabsence and the presence of α specific inhibitor,BuPdGTP,or its monoclonal antibody.The levels of DNApolymerase δ were determined in H · A fractions after separating it from the other two enzymes.The levelsof DNA polymerase ε were identified in H · A fractions by the use of α-monoclonal antibody or BuPdGTP.Results showed that in the mouse forebrain DNA polymerase α,δ,ε activities are the highest before birth,decline sharply following birth and are very low on the 8th day and hardly detectable on the 17th day;as forthe mouse spleen,however,DNA polymerase α,δ,ε activities are the lowest at birth,increase rapidly afterbirth and reach their maxima on the 8th day and then decline gradually but remain in higher levels.Theseresults not only prove that DNA polymerase α and δ take part in cell DNA replication but also suggest thatDNA polymerase ε is involved in DNA replication.
The levels of DNA polymerase α,δ,ε were examined in the neonatal mouse forebrains and spleens.The levels of DNA polymerase α were determined by the difference of polymerase activity in the absence and the presence of α specific inhibitor,BuPdGTP,or its monoclonal antibody.The levels of DNA polymerase δ were determined in H · A fractions after separating it from the other two enzymes.The levels of DNA polymerase ε were identified in H · A fractions by the use of α-monoclonal antibody or BuPdGTP. Results showed that in the mouse forebrain DNA polymerase α,δ,ε activities are the highest before birth, decline sharply following birth and are very low on the 8th day and hardly detectable on the 17th day;as for the mouse spleen,however,DNA polymerase α,δ,ε activities are the lowest at birth,increase rapidly after birth and reach their maxima on the 8th day and then decline gradually but remain in higher levels.These results not only prove that DNA polymerase α and δ take part in cell DNA replication but also suggest that DNA polymerase ε is involved in DNA replication.
基金
the Basic Research Grant form Chinese Academy of Sciences
