一种新的生物效应调节剂——PSP——Ⅰ.PSP的药理学研究

A New Biological Response Modifier Substance-PSP—— I Pharmacological Research of PSP

本文报道作为生物效应调节剂的PSP对正常动物、负瘤动物和施用化疗药物小鼠的肿瘤免疫学研究结果。口投或腹腔注射PSP(0,5～2.0g/kg×9或200～400rug/Kg×4)能明显提高小鼠对印度墨汁的廓清率(P<0.01),其作用和刺五茄相似;口投1.5g/kg×5剂量的PSP可使小鼠产生IL-2量比生理盐水组高1倍(P<0.01);用含150～800ug/mL PSP的细胞培养液,在Con A存在下,能使牌T淋巴细胞增长1.5～4.0倍;用100～1000ug/mL PSP的细胞培养液培养人血白细胞,其诱生的。和Y干扰素量比对照高2～4倍;PSP用于注射环磷酰胺小鼠,其白细胞数有不同程度的回升,口投组(2.25～4.00g/kg)从40回升到69～74千/mm^3腹腔注射组(200～400mg/kg)从60回升到80～86千/mm^3,PSP还能消除环磷酰胺对IL-2的抑制作用,使注射环磷酰胺小鼠IL-2量从1557cpm上升到2374cpm(P<0.01);PSP对迟发超敏反应(DTH)也有类似效应,它能使被环磷酰胺抑制的DTH反应得到恢复(P<0.05)。 PSP用于负Sarcoma肉瘤小鼠,能使小鼠因负瘤而萎缩的胸腺重量得到增加(P<0.05)并能提高负瘤鼠的抗体和补体C_3含量(P<0.01)。

This text is a report on the results of the effects of PSP on the immunology of the norinal animals ,the tumor-bearing animals and the animals administered with chemotherapy and radiotherapy.Oral administration or abdominal injection of PSP(0. 5～2.0g/kg×9 or 200-400mg/kg ×4)can appreciably raise the mice' s clearance rate of the Indian ink (P<0. 01). Its function is similar to that of acanthopanax.The oral administration of 1. 5g/kg×5 of PSP can make mice produce an amount of IL-2 twice as high as that of physiological saline group (P<0. 01). Under the presence of Con A , the application of 100-800ng/mL cell cultivation solution of PSP can increase the T lymphocytes of the spleens by 1. 5 to 4 times. With the application of cell cultivation solution of PSP (100-1000mg/mL)to cultivate the WBC of human blood,the amount of a or γ interferon induced will be 2 to 4 times as high as that of the control group. The application fo PSP to mice injected with cyclophosphamide will have different degrees of increase of their numbers of WBC. The numbers of WBC of the oral administration group(2. 25～4.00g/kg×8)will rise from 41,000～69,000 up to 74,000/mm3 and those of the abdominal injection (200～400mg/kg)group will rise from 60, 000～80, 000 up to 86, 000/mm3. PSP can also remove the inhibitory effect of cy-clophosephamide on IL-2,making the amount of IL-2 of mice injected with cyclophosphamide rise from 1557 to 2374 cpm(P<0. 01). PSP has also the same effect on the delayed type of hypersensitive reaction (DTH). It can restore DTH reaction inhibited by cyclophosphamide. (P<0. 05)The application of PSP to sarcoma bearing mice can increase the weight of the thymus atrophied (P<0. 05) and can also increase the amount of antibody and complement C3of the sarcoma bearing mice (P<0. 01).