摘要
A rat model of pleurisy was established with an intrapleural injection carrageenan(Car) in the dose of 10 mg/kg to study the anti-inflammatory action of tetrandrine(Tet) and its mechanism. At the 8th hour after injection, there was an increase of cytosolic free calcium level in the neutrophils (Neu[Ca￣(2+]_i) and intensification of calmodulin activity of the neutrophils(Neu-CaMA) in the pleural exudate. When Tet was given intragastrically in the doses of 10, 20 , 40 and 80 mg/kg 30 min before and 4 h after the injection of Car, the amount, protein content and neutrophil count of the pleural exudate were significantly reduced, Neu[Ca2+]_i decreased and Neu-CaMA inhibited. In addition, calcium(32 μmol/L) was able to antagonize the inhibition of Tet(20～160 μmol/L) on Neu-CaMA in vitro. On the basis of these findings, it is believed that the excellent anti-inflammatory effect of Tet might be related to its antagonism to calcium-calmodulin system of the body and the inhibition of Tet on Neu-CaMA is mediated through the reduction of Neu-[Ca2+]_i.
