人参皂苷单体Rb1、Re及Rg1对肾上腺素所致小鼠耳廓微循环障碍的改善作用

Improvement of Ginsenoside Rb_1,Re and Rg_1 on mouse auricle microcirculation disorder induced by adrenaline

目的:观察人参皂苷单体Rb1、Re及Rg1对肾上腺素所致小鼠耳廓微循环障碍的影响。方法:昆明种小鼠40只分为4组(每组10只),对照组、人参皂苷单体Rb1组、人参皂苷单体Re组及人参皂苷单体Rg1组。除对照组外,各组按10mg·kg-1·d-1腹腔注射给药一次,对照组腹腔注射同体积的生理盐水。连续给药4d,末次给药30min时将小鼠固定于微循环观测分析系统显微镜下,观测小鼠正常及腹腔注射盐酸肾上腺素(1mg·kg-1)10、20和30min时小鼠耳廓微血管的管径、血流速度及毛细血管交叉网开放数目。结果:与对照组比较,人参皂苷单体Re组及Rg1组小鼠在腹腔注射盐酸肾上腺素(10、20和30min)小鼠耳廓微血管管径均明显扩张(P<0.05或P<0.01),微血管血流速度均明显加快(P<0.05或P<0.01),微血管交叉网开放数目均明显增加(P<0.05);Re组与Rg1组比较差异无显著性(P>0.05)。人参皂苷Rb1组对肾上腺素所致微循环障碍无显著影响。结论:人参皂苷单体Re及Rg1对肾上腺素所致小鼠微循环障碍具有明显的改善作用。

Objective To observe the effect of Ginsenoside on mouse auricle microcirculation disorder induced by adrenaline. Methods Forty mice were equally divided into 4 groups （n=10）：control group, Ginsenoside Rb1 treated group, Ginsenoside Re treated group and Ginsenoside Rg1 treated group. Ginsenoside Rb1 , Re or Rg1 were separately given intraperitoneally in the mice per day at dose of 10 mg·kg^-1 for 4 d. The microcirculation disturbance in mice was brought by rapid injection of adrenaline. The micro vascular diameter, the blood flow speed and micrangium crossing net patefaction number in auricle of mice at normal condition and in the 10, 20, 30 min after injection of adrenaline were observed by microeirculation microscope with analytical system. Results Compared with control group, Ginsenoside Re or Rg1 could expend the narrower diameter of the mieroangium （P〈0.05 or P〈0.01）, quicken the slower blood flow speed induced by adrenaline （P〈0.05 or P〈0.01）, and increase the micrangium crossing net patefactiorr number （P〈0.05）. Ginsenoside Rb1 possessed no significant effect on microeirculation disturbance by adrenaline. Conclusion Ginsenoside Re or Rg1 can relieve the contract of blood vessels obviously induced by adrenaline.