雌激素受体α和β在人正常乳腺组织中的表达及意义

Expression of ERα and ERβ in normal human mammary gland and significance

目的:观察雌激素受体α(ERα)和β(ERβ)在人正常乳腺组织细胞中的定位,为探讨ERα和ERβ与乳腺组织生长发育的关系奠定基础。方法:应用免疫组织化学的方法,检测16例在加拿大拉瓦尔大学附属医院接受乳房缩小成形术患者的正常乳腺组织的ERα和ERβ阳性表达率及分布情况。结果:ERα在小叶上皮细胞和导管上皮细胞的细胞核内检测到,在小叶和小叶内导管分布于上皮细胞的内层,在小叶间导管分布于上皮细胞的外层。ERβ在小叶上皮细胞和导管上皮细胞的细胞核内检测到,但是ERβ在上皮细胞中的分布并无规律,而且在细胞浆内也可以检测到弱到中等强度的染色。同时,在一些间质细胞、血管内皮细胞和淋巴细胞亦出现染色情况。ERβ在乳腺上皮细胞中阳性百分数明显高于ERα的阳性百分数,两者比较差异有显著性(t=29.789,P<0.01)。同时ERβ的阳性表达率明显高于ERα的阳性表达率,两者比较差异有显著性(χ2=8.127,P<0.05)。结论:雌激素受体各亚型在人正常乳腺组织中有着不同的分布特点,ERβ的广泛分布暗示着ERβ可能是乳腺组织中占优势的雌激素受体。

Objective To explore the cellular location of ER subtypes （ERα and ERβ） in normal human mammary gland and provid the foundation for exploring the relationship between ERα , ERβ and development of normal mammary gland. Methods Using immunohistochemistry, the expressions and cellular distribution of ERα and ERβ in the normal tissues of 16 patients who had accepted reduction mammoplasty specimens in the Affiliated Hospital of University Laval were detected. Results ERα immunoreactivity was detected in the nuclei of epithelial cells lining lobules and ducts. The positive cells located in the inner layer of the two epithelial layers in the lobules and intralobular ducts, but in the interlobular ducts the positive cells located in the outer layer. Although ERβ was also seen in these cells, there was irregularity of its distribution. Weak to moderate cytoplastic staining of ERβ in epithelial cells of lobules and ducts were fonud. Occasional nuclear staining was seen in the stromal cells, endothelial cells and lymphocytes. The percentage of ERβ positive expression in the epithelial cells was much higher than that of ERα, there was markedly difference between them （t=29.789, P〈0.01）. The positive expression rate of ERβ in the mammarv gland was much higher than that of ERα, there was markedly difference between them （X^2=8. 127, P〈0.05）. Conclusion ER subtypes have distinct distribution patterns in the normal mammary gland. The widespread distribution of ERβ suggests that it may be the dominant ER in the mammary gland.