白枕鹤精液冷冻技术的研究

A study on semen freezing in Grus vipio

白枕鹤（ＧＲＵＳＶ ＩＰＩＯ）羽毛青灰，红面，体型健美优雅，具有很高的观赏价值，已被国家列为二级保护动物。对白枕鹤类的人工授精、孵化、育雏以及冷冻精液技术的研究，可促进人工饲养条件下鹤类的增殖和种群的繁衍。本研究采用Ｂｅｌｔｓｖｉｌｌｅ稀释液做为基础液，４—６％ＤＭＳＯ做为冷冻保护剂，按常规冷冻法冻精配种受胎率达４０％，此项研究填补了我国在鹤类精液冷冻技术方面的空白。

Grus vipio,a kind of birds,which has dark grey feather,a red face and a graceful conformation,is of high ornamental value and is one of the endangered species under the national protection of second class. Research on artificial insemination and semen freezing and artificial hatching and starting in Grus vipio will promote the reproduction of the crane under artificial breeding. In this experiment,semen was frozen following a routine procedure with Beltsville extender as basic solution and 4-6 % DMSO as cryoprotectant,and a conception rate of up to 40% was obtained after artificial insemination with the thawed semen. This result has filled in the gaps in the technique for crane semen freezing in China.