猪微注射基因导入系统影响受体受孕率及产仔率的几个因素

Factors influencing pregnancy and farrowing rates of the recipient sows in the gene transfer program

在应用显微注射、胚胎移植系统技术，进行猪ＯＭＴ／ＰＧＨ基因导入的研究中，对移入受体的胚数，ＰＭＳＧ处理受体以及不同的移植方法（自体移植与异体移植）等影响受体受孕率及产仔率的因素，进行了试验分析，结果表明：（１）移入受体的注射胚数分别为１０—１９枚、２０—２９枚、３０枚以上时，其受孕率为４５．５％、６４．７％和７１．４％，产仔率为８．０％、１９．６％和１４．５％，以移入２０—２９枚效率最高；（２）用ＰＭＳＧ对受体母猪做同期发情处理，其受孕率和产仔率比选择自然发情的受体分别下降２５％和８．５％；（３）采用自体移植的方式，在移入胚数基本相同的情况下（１５枚左右），比异体移植的受孕率和产仔率分别提高１４．３％和６．４％。

In the experiments of OMT/PGH gene transfer in swine, involving microinjection and embryo transfer procedures. the factors which affect pregnancy and farrowing rates in the recipients were studied. The results are as follows: (1)when the number of gene transplant embryos transferred to each recipient was 10-19, 20-29,and more than 30,the conception rates were 45. 5%, 64. 7 %and 71. 4%,and the farrowing rates were 8. 0%, 19. 6 %,and 14.5%, respectively, with the highest farrowing rate being obtained when the recipients received 20-29 embryos each. (2)when recipients were treated with PMSG for synchronization of estrus, their pregnancy rate and farrowing rate were reduced by 25% and 8.5%, respectively, compared with those of the natural estrous recipients. (3)The pregnancy and farrowing rates in the homotransfer (in which embryos were transferred back to their donors)recipients were 14. 3% and 6. 4% higher than those in the heterotransfer recipients.