急性白血病流式细胞计DNA含量测定及其意义

Acute Leukemic Patients DNA Flow Cytometric Analysis and Clinical Implications

本文用流式细胞计对34例成年初治急性白血病人,16例完全缓解病人及20例骨髓大致正常者进行了骨髓细胞DNA含量测定,并结合临床进行了探讨.结果表明:初治病人骨髓细胞G_1/G_0期77.49±7.76％,S期13.91±5.93％,G_2/M期3.75±1.85%.S期比值在急淋病人和急非淋病人之间无显著差异(P=0.8)。初治病人骨髓细胞S期比值低于缓解后病人(15.91±2.75)及骨髓大致正常者(16.42±3.52).治疗前S期比值低时缓解率低,诱导化疗时间长,但缓解后维持时间较长;S期比值高缓解率高且总体生存期长.非整倍体出现率32％,这类病人诱导化疗效果较好.

Flow cytometric analysis of bone marrow cellular DNA content was performed in 34 newly diagnosed patients with acute leukemia, Hi remission patients and 20 approximately normal bone marrow specimens from non-leukemic patients.The relationship between the findings and clinical aspects was also investigated.The resutls showed.In the newly diagnosed patients the G_1/G_0 phase percent was 77.94±7.67%; the S-phase percent 13.91±5. 93% and the G_2/M phase percent 3. 75±1.85%. No difference was found between ANLL and ALL in the S-phase percent (p=0.8). S-phasc percent in remission patients and approximately normal marrow was higher than that of the newly diagnosed patients. The patients with lower S-phase percent correlate with lower remission rate and longer inductive therapeutic duration, but had longer remission duration compared to the patients with higher value.Higher S-phase percent at pretreatment predicted higher remission rate and longer survival duration.The incidence of ancuploid was 32%, the patients with aneuploid were easier to response to the inductive therapy.