高效液相色谱法测定孕尿雌三醇

Determination of Estrio1 in Pregnancy Urine By High Perfomance Liquid Chromatography

本文介绍孕尿雌三醇的分析,方法包括:200μl尿样酶解、分离柱提取以及用甲醇/磷酸盐缓冲液为流动相的反相液相色谱法定量。回收率95.5％、变异系数CV=3.25％(16.1mg/L n=4)。方法快速,简便、可靠,试剂消费少,应用于中晚期妊娠效果良好。

We describe an assay for estriol in pregnancy urine. The method involves enzymic hydrolysis and extraction by Sep-pak C_(18) cartridge and quantitation by reversed-phase liquid chromatography with a mobile phase consisting of methanol/phosphate buffer. Analytical recovery is 95.5%. The precision(CV) is 3.25％at 16.1mg/L(n=4). This assay is simple, fast reliable and inexpensive for reagents. It shows the effect on using this method in middle and late pregnancy.