检测麻风抗体ELISA用封闭剂的系统研究

Study of Blocking Agents in ELISA For Antibodies to Leprosy

本文探讨了蛋白质类封闭剂在麻风抗体检测ELISA中的封闭效果和以P/N比和t测验做为评价效果的指标,比较了不同封闭对同一抗原,同一封闭剂对不同抗原以及同一封闭剂不同浓度时的封闭效果。结果表明受试的各封闭剂在受试条件下以P/N为指标均显示了可靠的封闭效果(P/N>10),按P/N比大小,其顺序为:10％BSA(193.0)10％SM(146.0),20％EA(97.5)5％EAC(26.1),10％NGS(22.8),10％EA(18.0),5％BSA(16.0)。对两种廉价的封闭剂SM和EA以P/N比和t测验为指标进一步研究表明EA浓度在10％,15％和20％,SM浓度在5％和10％显示最佳结果。全面比较封闭效果、价格高低、贮运、来源难易等条件后认为SM,EA应作为首选封闭剂推广应用,特别是后者更符国情。

The authors compared systema- tically the efficacies of different blocking agents in ELISA for detec- ting antibody in sera of leprosy patients using P/N and t-test as evaluation indicators. The results indicated that : 1. with P/N ratio, all the blocking agents used showed reliable blocking efficacy, i. e. P/N >10, with following ordor :10% BSA (193.0),10% SM (146.0), 20%, EA (97.5) ,5%EAC (26.1),10% NGS (22.8) , 10% EA (18.0) and 5% BSA (16.0) ; 2. 10%, 15%and 20%EA, and 5% and 10% SM, both the blocking agents are inexpensive,and sho wed the best blocking results when the eva- luations were conducted with combi- nation of P/N ratio and t-test. SM and EA are recommended for use as blocking agent because they are easier to prepare and do not re-quire lowtemperature for shipping and stor- age.