CCl_4诱发大鼠肝硬化中纤维联接蛋白演变的免疫电镜观察

ELECTRON IMMUNOHISTOCHEMICAL MICROSCOPE OBSERVATION IN RAT CIRRHOSIS INDUCED BY CCL_4

应用免疫电镜对四氯化碳诱发大鼠肝硬化中纤维联接蛋白的演变进行了定位研究,发现在正常肝组织内纤维联接蛋白主要位于血窦内皮细胞表面、肝细胞血窦缘的微绒毛表面和Disse间隙内。在肝硬化发生的早、中期纤维联接蛋白在Disse间隙内增加显著,电镜下可见位于Disse间隙内的过渡细胞;在后期部分肝窦毛细血管化形成,纤维联接蛋白在Disse间隙内沉积减少。结果表明,纤维联接蛋白在Disse间隙内沉积可能影响贮脂细胞产生胶原,并介导胶原在该部位沉积增多,致肝窦毛细血管化,这是肝功减低的主要原因之一。

Localization of fibronectin was studied in rat cirrhosis induced by CCL_4 with electron immunohistochemical microscope. It was observed that fibronectin located in the space of Disse, the surface of endothelium and the surface of microvilli of hepatocytes. At the early and middle stage of cirrhosisgenesis, fibronectin increased remarkably in the space of Disse. Under electron microscope transitional cells were observed. When capillarization of sinusoids was formed, fibronectin deposition in the space of Disse, having effects on. collagen production of fatstoring cells and increasing collagan deposition, may be the crucial event in the cirrhotic process. The capillarization of sinusoids may have profound effect on hepatic systemic functions.