摘要
Passive immunization of female mice by the intraperitoneal administration of ascitic fluidscontaining monoclonal antisperm antibodies blocked the development of mouse zygotes tomorulae and blastocysts when cultured in vitro.Radioiodinated YWK-I monoclonal anti-bodies were prepared and administered intraperitoneally to Jemale mice.Significant radioac-tivity was found in washes of the oviductal lumens.The results suggest that antispermantibodies block fertilization and may have a deleterious effect on early developing embryos.
Passive immunization of female mice by the intraperitoneal administration of ascitic fluids containing monoclonal antisperm antibodies blocked the development of mouse zygotes to morulae and blastocysts when cultured in vitro.Radioiodinated YWK-I monoclonal anti- bodies were prepared and administered intraperitoneally to Jemale mice.Significant radioac- tivity was found in washes of the oviductal lumens.The results suggest that antisperm antibodies block fertilization and may have a deleterious effect on early developing embryos.
基金
Supported by Rockefeller Foundation Grant and Mellon Foundation Grant
