间日疟间接荧光抗体试验观察者变异与正常值上限研究

OBSERVER'S VARIATION AND UPPER LIMIT OF NORMAL VALUE:STUDIES ON INDIRECT FLUORESCENT ANTIBODY TECHNIC FOR VIVAX MALARIA

将间日疟阳性血清作系列倍比稀释进行间接荧光抗体试验,不同观察者平行读数或同一观察者先后读数的终点滴度之变异在一个倍比稀释度之内。对无疟疾感染史的正常人群522份血清作间日疟间接荧光抗体试验,其荧光抗体滴度正常值上限按99%位点估计为<1:10。

Indirect fluorescent antibody technic(IFAT)for plasmodiumvivax(P.V.)was performed on serial 2-fold dilutions of positive serum from a pateint with vivax malaria.The variation of reading between two observers or two observations on end- point of IFA titer was limited at the range of 2-fold dilution.IF AT for p.v.was carried out on sera from 522 subjects from area no malaria case had been reported since 1972.The result shows that<1:10 is estimated as 99% percentile of upper limit of normal value of IFA titer.