急性犬心肌缺血后冠脉再灌注损伤 Ⅱ、心肌琥珀酸脱氢酶光镜及电镜组织化学研究

Coronary Artery Injury to Postischemic Canine Myocardium.

近年来,许多学者进行了早期心肌缺血后冠脉再灌注的研究.有人认为冠脉再灌注有利于早期缺血心肌的恢复;我们的实验研究表明,急性心肌缺血后单纯冠脉再灌注不仅能使缺血心肌组织结构的损伤加重,而且能使原有的心肌梗塞范围扩大,这与一些国外学者的研究结果是一致的.心肌中的琥珀酸脱氢酶是心肌进行有氧代谢的一种重要的酶.本文应用的酶组织化学方法研究了急性犬心肌缺血及缺血后冠脉再灌注对此酶浮性和分布的影响,目的在于进一步观察急性心肌缺血后冠脉再灌注的效果等问题.

To further demonstrate reperfusion injury to post-ischemic canine myocardium, the present experiments were performed to investigate the effecof myocardial ischemia on activity and localization of succinic dehydrogenase(SDH) in the groups of occluding left anterior descending coronary artery for 60 and 90 min. followed by reopening for 120 min. by enzyme histochemistry and cytochemistry. SDH activity decreased in the group of coron ary occlusion for 60 min., especially for 90 min. Reperfusion for 120 min. resulted in much decrease of SDH activity and chaotically extended distribution. In conclusion, reperfusion causes much more severe injury to the postischemic canine myocardium, i.e, reperfusion injury, which may be, considered as one of the leading causes of cardiac death in forensic pathology.