上海人群红细胞EAP、PGD、ADA和AK同步电泳检测表型的分布及血痕的研究

The Phenotype Distribution of the Red Cell EAP, PGD, ADA and AK in Shanghaiese with reference to bloodstain detection

红细胞酸性磷酸酶(EAP)、6-磷酸葡萄糖酸脱氢酶(PGD)、红细胞腺苷脱氨酶(ADA)和腺苷酸激酶(AK)首先被(HOPKinson(1963)等用淀粉凝胶电泳分析法证明它们的遗传多态性以来,作为遗传标记受到人类遗传学及法医学工作者注意,国内近年来也陆续开展红细胞酶型的分型并将其应用于亲权鉴定及个体识别.为了调查上海地区ADA、EAP、PGD和AK的表型频率和基因频率,探索这四种酶型在法医学上的应用,我们采用水解淀粉和琼脂糖混合凝胶为支持介质,同步电泳检测上述四种红细胞酶型和表型,获得较满意的结果.

The phenotype distribution of human red cell EAP, PGD, ADA and Ak in Shanghaiese was studied using starch gel simultaneous electrophoresis. Among 537 fresh blood samples, the freqnencies of EAP phenotypes were: EAP B74.3%, EAP BA 21.4%, EAP A 3.9%, EAP CB 0.4%, EAP CA 0% and EAP C 0%. Among 358 fresh blood samples, the freqnencies of ADA phenotypes were: ADA 1-1 83%, ADA 2-1 16%, ADA 2-2 1%. Among 385 fresh blood samples, the freqnencies of PGD phenotypes were: PGD 1-1 78%, PGD 2-1 21%, and PGD 2-2 1%. Among 296 fresh blood samples, the freqnencies of AK phenotypes were: AK 1-1 100%. This paper described a few improvements compared with conventional method. And we studyed that some blood stain samples could keep how long time under room temperature on 4 isoenzymes.