促进皮肤创面愈合及抗感染作用的实验研究

Experimental Study on the Actions of the Moist Burn Ointment on Promoting Healing of Skin Wound and Anti-infection

湿润烧伤膏是湿润暴露疗法所采用的药物,也是湿润暴露疗法的精髓。我们在临床初步应用中,感到该药物有较明显的促进组织修复及控制局部感染的作用。为进一步了解药物对皮肤愈合及抗感染的作用及可能机理,我们进行了下面的实验观察。

3 aseptic incisions, with same diameter and same depth, were made in the skin of rabbit and applied with drug, single ground substance and blank control respectively. The former two were applied at definite times. One rabbit was killed on the 4th, 8th days respectively and the tissue from the 3 wounds was taken for pathological exami-nation. As the wound healed, the areas of scar were drawn and calculated against standard chart. Meantime, in vitro bacteriostatic examination was made by adopting agar culture and plate drilling method. It was observed that the reaction in the wounds of the moist exposed group was earlier than the other two groups (P<0.05), manifesting heal promoting action on wounds. Histologically, it revealed that the Moist Burn Ointment stimu-lates the regeneration of blood vessel in the granulation tissue and its blood circulation, thus promotes abundant oxygen and nutrient supply and metabolism process. Clinically, it reveals satis-factory anti-infection action, having been verified by our experiment. Strangely, in vitro experiment showed that it has no bacteriostatic action on staphy1ococcus aureus, escherichia coli, pseudomonas aeruginosa. It was thus suggested that its clinical effect of anti-infection might be realized through the actions of formation of free local drainage and the strengthening of immune mechanism. In the paste group, less collagenous fibers were found in the granulation tissue, this suggests that the paste possesses the actions of inhibiting collagen growth, coordinating the ratio of its growth with epithelial cells, thus decreases the formation of scar tissue.