免疫印斑试验在血吸虫病现场诊断中的应用

A FIELD APPLICATION OF IMMUNO-BLOT IN DIAGNOSIS OF SGHISTOSOMIASIS JAPONICAM

本文报告用免疫印斑试验(immunoblot)以成虫31/32kD诊断蛋白检测52例血吸虫病患者血清抗体,结果阳性率为100％,而20例健康人均无反应。同时对肺吸虫病患者36例,旋毛虫病50例和囊虫病26例进行交叉反应测试,结果均无交叉反应出现.以免疫印斑试验与间接血凝试验(IHA)和酶免疫吸附试验(ELISA)对上述血清进行测试比较,结果后两种方法均出现不同程度的交叉反应,个别高达58.3％,显示了免疫印斑试验的优越性和诊断价值. 应用此法对流行区进行现场人群调查,136例中抗体阳性率为58.1％,对两项血清学检查均为阳性反应的65例,再进行粪检,结果24例虫卵阳性. 该法敏感、特异,重现性好,是一种新的血吸虫病血清学诊断方法,现将固相酶免疫试验常规法改进为微量法并制成试剂盒供基层使用。

Immuno-blot was used in diagnosis of schistosomiasis japonica using diagnostic 31/32 KD proteins in S.japonicum Among 52 confirmed schistosemiasis cases 100% showed positive reaction while none of the sera of 20 normal control reacted. No cross reactions occured with sera from 36 paragonimiasis; 26 cysticercosis and 50 trichinelliasis cases. However, 11.5-58.3%cross reactions were found with above sera in IHA and ELISA. The diagnostic value of immuno-blot and 31/32 kD schistosome proteins were confirmed. A field application of immuno-blot in diagnosis of schistosomiasis japonica was performed. The positive rates in immuno-blot and IHA were 58.1% and 54.4% respectively in 136 cohorts with and without past history of schistosomiasis in the endemic area. It was found that immuno-blot was highly sensitive, specific and reproducible.It was easy to perform and suitable for field use.