日本血吸虫病免疫酶染色试验的影响因子及诊断价值研究

DIAGNOSTIC VALUE AND FACTORS EFFECTING OF IMMUNOENZYMATIC STAINING TEST(IEST) FOR SCHISTOSOMIASIS JAPONICA

以感染血吸虫后7周的鼠肝组织内虫卵及6周的病鼠门静脉内血吸虫成虫的冰冻切片(TEFS及AWFS)抗原作免疫酶染色试验最为适宜,最适切片厚度均为7μm。TEFS的抗原性显著地高于AWFS及感染鼠肝石蜡切片.TEFS保存于4℃中3个月的抗原反应性下降不显著,保存于-20℃中8个月的抗原反应性不减,用4％H_2O_2溶液处理TEFS,可消除鼠肝组织内源性酶的非特异性染色。不适合的试验血清量、血清孵育时间、酶结合物作用时间、试液离子强度及酸碱度均能影响反应结果。应用TEFS抗原作免疫酶染色试验(TEFS—IEST),以诊断血吸虫病人,呈现较高的敏感性(96.7％),特异性(98.7％)及较低的交叉反应率(0～3.3％),其敏感性显著地高于AWFS—IEST及COPT。TEFS—IEST应用抗原经济、检测高效及获得结果较快,值得扩大应用。

The most suitable antigenic frozen sections are the sections of infected mice liver and adult worm of Schistosoma japonicum at 7th week and 6th week after infection respectively; the most suitable thickness of section is 7μm. The antigenicity of liver tissue-egg frozen section(TEFS) is markedly higher than that of adult worm frozen sectin(AWFS) and of liver tissue-egg paraffin embedded section.TEFS, as antigen, is considered to be of high sensitivity(96.7%), high specificity (98.7%) and lower cross reaction rates(0~3.3%) in IEST for diagnosis of schistosomiasis. The sensitivity of TEFS-IEST ig markedly higher than that of AWFS-IEST and that of COPT. The main advantages of TEFS-IEST are lower cost of the antigen, high sensitivity and rapid yield of results.