应用单克隆抗体酶联免疫吸附试验双抗体法检测肺吸虫病循环抗原

DETERMINATION OF CIRCULATING ANTIGENS IN HUMAN PARAGONIMUS WESTERMANI BY MEANS OF A DOUBLE SANDWICH METHOD ENZYME-LINKED IMMU-NOSORBENT ASSAY (DS-EAISA) USING MONOCLONAL ANTIBODIES

本文应用兔抗囊蚴抗原的单克隆抗体(McAb)酶联免疫吸附试验(ELISA)双抗体法检测38例人体肺吸虫病循环抗原,其阳性率为100％(38/38)。

A DS-ELISA using rabbit anti-metacercaria serum-immunoglobulins as the first antibody and anti-metacercaria monoclonal antibodies as the second antibody were developed for the determination of circulating antigens in 38 cases of human paragonimiasis. The positive rate was 100% (38/38).