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ENRICHMENT OF THE SPINDLE FIBER ATTACHMENTS FROM MOUSE LIVER CELL NUCLEI

ENRICHMENT OF THE SPINDLE FIBER ATTACHMENTS FROM MOUSE LIVER CELL NUCLEI
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摘要 Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouselivers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and highspeed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured byradioimmunoassay. The basic method offers opportunities for further increases of yield andfor the enrichment of SFA uf other vertebrates. Spindle fiber attachments (SFAs) were enriched in a fraction of nuclei isolated from mouselivers. The enrichment method combines sonication, treatment with 2 mol/L NaCl and highspeed centrifugation. SFA was enriched 27-fold on the average (n = 4) when measured byradioimmunoassay. The basic method offers opportunities for further increases of yield andfor the enrichment of SFA uf other vertebrates.
出处 《Science China Chemistry》 SCIE EI CAS 1989年第7期816-821,共6页 中国科学(化学英文版)
关键词 SPINDLE FIBER ATTACHMENT ENRICHMENT isolated liver NUCLEI mouse spindle fiber attachment enrichment isolated liver nuclei mouse
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