摘要
In this paper, the increase of cellular cAMP and cGMP levels in macrophages induced bypppA2’p5’A2’p5’A （briefly 2’-5’P<sub>3</sub>A<sub>3</sub>） is first reported. The optimal concentration of 2’-5’P<sub>3</sub>A<sub>3</sub> for the elevation of cellular cGMP to the highest level is 10<sup>-7</sup>-10<sup>-6</sup>mol/L, while thatfor cAMP is 10<sup>-7</sup>mol/L. The time for cGMP to reach its peak value is 15 min and that forcAMP is 2 h, when the cells are treated with 2’-5’ P<sub>3</sub>A<sub>3</sub> at 10<sup>-7</sup>mol/L, which is the optimalconcentration for developing biological effect of macrophages （phagocytosis）. These resultssuggest that cGMP and cAMP may be related to, or may be the mediators for, 2’-5’P<sub>3</sub>A<sub>3</sub>action.
In this paper, the increase of cellular cAMP and cGMP levels in macrophages induced bypppA2'p5'A2'p5'A (briefly 2'-5'P_3A_3) is first reported. The optimal concentration of 2'-5'P_3A_3 for the elevation of cellular cGMP to the highest level is 10^(-7)-10^(-6)mol/L, while thatfor cAMP is 10^(-7)mol/L. The time for cGMP to reach its peak value is 15 min and that forcAMP is 2 h, when the cells are treated with 2'-5' P_3A_3 at 10^(-7)mol/L, which is the optimalconcentration for developing biological effect of macrophages (phagocytosis). These resultssuggest that cGMP and cAMP may be related to, or may be the mediators for, 2'-5'P_3A_3action.
基金
the National Natural Science Foundation of China.
