摘要
维生素D<sub>3</sub>在肝脏羟化酶的作用下可转化为25—OH—D<sub>3</sub>。在体外,以<sup>3</sup>H—D<sub>3</sub>为底物,应用佝偻病鸡和正常牛肝脏的微粒体悬液（含25羟化酶）与孵化因子（含O<sub>2</sub>、Mg、NADpH等）一起作用于底物使其向<sup>3</sup>H—25—OH—D<sub>3</sub>转化。结果,正常十肝微粒体悬液促使的羟化反应有9％的转化率,而佝偻病鸡肝微粒体悬液促使的羟化反应有70％的转化率。维生素D<sub>3</sub>体外羟化反应的研究,将为生物学方法制备1,25—（OH）<sub>3</sub>—D<sub>3</sub>甾族激素和酶动力学深入研究奠定基础。
The production of 25-OH-D3 from D3 is catalyzed by 25-hydroxyr lase in liver microsomes. The 3H-D3 were converted in vitrointo 3H-25-OH-D3 by the microsomes suspension which contains liver 25-hyd-roxylase of rachitic chick and healthy ox, and the mixture of molecular oxygen, Mg and NADPH. The result showed that the conversion rate is 9% in the microsomes suspension of healthy ox and 70% in that of rachitic chick;, The study of hydroxylation reaction in vitro will lay a foundation for praparation of 1, 25,- (OH) 2-D3 hormone and research on enzyme kinetic.
出处
《牡丹江医学院学报》
1989年第3期131-133,共3页
Journal of Mudanjiang Medical University
