人肺癌DNA转化活性的初步研究

PRELIMINARY STUDY ON TRANSFORMING ACTIVITY OF DNA FROM HUMAN LUNG CARCINOMA TISSUE

本文用磷酸钙沉淀技术,以人肺鳞癌组织高分子量 DNA 进行了小鼠成纤维细胞NIH3T3的转化实验。得到的转化细胞能在软琼脂板上生长;可诱导 NIH3T3细胞二轮转化;在裸鼠体内有明显的致瘤性;其 DNA 经酶切后与人 ALU 重复序列探针杂交呈阳性反应;并证明有 c～myc 基因扩增及有 v-fos 同源宁列的存在。

Transformation of NIH3T3 cells with high molecular weight DNA isolated from human squa- mous cell carcinoma of lung,was performed by the calcium phosphate precipitation technique.The morpho- logically transformed cells could grow in colony on soft agar.The DNA isolated from primary transformants could induce second round transfection.These transformants were tumorigenic in nude mice.Hybridization of DNA from transformants digested with restriction endonuclease EcoRI with human ALu repeat sequence was positive.Amplification of c-myc gene and DNA sequence homologous to v-fos gene were found in these transformants.