人α_1型干扰素工程菌表达质粒拷贝数的研究

The Copy Number of Expression Plasmid in Engineered Strain for Producing HulFNα_1

本文采用荧光测试技术对人a_1型干扰素工程菌在高密度发酵条件下表达质粒拷贝数进行了测定,并对其表达量和菌体生长进行了研究,结果表明在高密度发酵条件下工程菌的表达质粒拷贝数十分稳定,约为每细胞100拷贝左右,为摇瓶发酵的1.6倍。本文还研究了发酵过程中菌体密度和表达量的关系。

Under different growth conditions, the copy number of expression plasmid in IFN伪_1 producer BMH71-18/pBV867 was determined by fluorescence technique. The results showed that the copy number of plasmid in IFN伪_1 Producing strain was stable at about 100 copies/cell during high density fermantation. It is about 1.6 fold of that in the cell, which were grown in the shaking flask.The relationship between cell density and expression level in the course of fermantation was discussed.