应用酶免疫分析法测定PreS2-HBsAg中的pHSA-受体

Detection of pHSA-receptor of PreS2-HBsAg by EIA

我们应用酶免疫分析法测定了 PreS2-HBsAg 中的 pHSA-受体。该法具有较好的特异性和重复性,并发现经 Sephadex G-200层析与未经过层析的 pHSA,测得 pHSA-受体结果无差异。用该法分别检测重组蚕核多角体病毒的细胞培养上清及超声波处理的培养液,后者 PreS2-HBsAg 的 pHSA-受体浓度较前者提高3倍。

EIA was established to derect the pHSA-receptor titre of PreS2-HBsAg. The experimental results demonstrated that EIA had good specificity and reproducibil- ity.No significant difference Was found whether the pHSA was subjected to Sephadex G-200 chromatography or not.APPlying this method to detect the expressed products (PreS2-HBsAg pHSA-receptot)by recombinant Bombyx moti nucIear polyhydrosis virtts,both in cell culture supernatant and in ultrasonicated cell suspension,the expre- ssion 1eveI WaS higher in the latter.