发色底物测定法在凝血与抗凝血中的应用

The Application of Chromogenic Peptide Substrate Method in Determing Coagulation and Anti-coagulation

用发色底物法测定多种疾病的PK∶A、FX∶A、ATⅢ、A、H-ATⅢ、PLG∶A及α_2-PL∶A等.结果表明,低凝状态病(肝硬化、血友病),血浆凝血因子减低,抗凝血因子和纤溶活性减低或正常;高凝状态病(脑血栓、妊高征),血浆凝血因子和纤溶活性减低或增高,

In this study, plasma prekallikrein activity (pk:a), factor X activity (FX:A) , antithrombin Ⅲ activity (ATⅢ:A) , heparin-AT Ⅲ complex plasminogen activity (PLG:A) and α_2-plasmin inhibitor activity (α_2-PI:A) were assessed by chromogenic peptide substrate (CPS) method in patients with various kinds of diseases. The results have shown that in hypocoagulable state diseases (cirrhosis, hemophilia) plasma coagulable factor levels were lower than those in normal subjects, but the values of plasma anticoagulable factors and the activity of fibrinolysis were lower than or similar to those in normals. In contrast, in bypercoagulable state diseases (cerebrovascular thrombosis, pregnancy accompanied with hypentension) , the plasma coagulable factors levels were higher than those in normals, but the values of plasma anticoagulable factors and theactivity of fibrinolysis were normal or high.