4℃保存血标本和血循环FI对IAb测定值的影响

EFFECT OF STORING BLOOD SAMPLE AT 4℃ AND FI IN BLOOD CIRCULATION ON IAb CONCENTRATION

本文报导注射胰岛素后不同时间血循环游离胰岛素(FI)和血标本4℃保存对胰岛素抗体(IAb)测定值影响的研究。结果提示,不同采血时间,血循环FI与IAb值呈负相关,4℃保存血标本可提高IAb测定值,但结果不恒定,保存多天再进行测定亦不易为临床上采用。而血标本经酸化抽提,所测定的IAb值稳定,后者在临床和科研中更为有用。

This study focuses on investigating the effect of free insulin (FI) in blood circulation after subcutaneous injection of regular insulin and of storing blood samples at 4℃ on insulin antibody (IAb) measured.The negetive correlation between IAb and FI was significant.The author believe that blood samples collected at regular time and aged at 4℃ gave a higher IAb concentration, but the results are unconstant and it is not convenient for clinic to measure IAb in blood sample after they have been stored for several days.The results obtained from extracted serum are constant.It is of great singnificance in clinic and laboratory work.