胃蛋白酶、胰蛋白酶、牛磺胆酸钠对卫氏并殖吸虫囊蚴脱囊影响的研究

STUDY ON THE.IMPACT OF PEPSIN,TRYPSIN AND SODIUM TAUROCHOLATE ON THE EXCYSTATION OF PARAGONIMUS WESTERMANI

本文采用正交设计,模拟卫氏并殖吸虫囊蚴在宿主体内的消化脱囊过程,以胃蛋白酶及其作用时间,胰蛋白酶和牛磺胆酸钠为可能影响囊蚴脱囊的因素,进行了体外脱囊试验。首次提出胃蛋白酶及其作用时间对卫氏并殖吸虫囊蚴脱襄的重要影响,并提出,在体外37℃条件下,囊蚴置于0.025％胃蛋白酶溶液中作用10min,再置于0.025％牛磺胆酸钠溶液中作用,最有利于囊蚴中后尾蚴的脱囊。

The excystation of Paragonimus westermani metacercariae were experimentally obser- ved in vitro.The results show that pepsin and its action time on the parasite play an important role in the excystation of the metacercariae under the condition of 37℃ ia the 0.025％ pepsin solutioa for 10 minutes.