摘要
固定化酶的制备及其性质己在世界范围内引起人们的极大兴趣,而这种兴趣主要的体现在它们的工业生产方面。NAD(P)H再生是进行酶促合成及氧化还原反应的重要限制性因素之一。已有大量利用酶学的、电化学的、固定化叶绿体及固定化微特征细胞方法进行NAD(P)H再生的报道。在这些方法中,酶学方法由于它们原理简单、反应器造价也不太昂贵己被应用于工业生产之中;固定化微生物细胞虽然也被用于工业生产,但它们反应的选择性较差且反应器的造价也较前者为高;虽然电解法和固定化叶绿体有可能用于NAD(P)H的再生,但直到目前为至仍未见到有关实际应用的报导。
Considerable worldwide concern has arisen in preparation and properties -of immobilized enzymes and it has been applied to their rndustrial production. Regeneration of NAD(P)H is one of the important limiting factors for earring out enzymatic synthesis and oxidoreductive reactions. Numerous enzymatic, electrochemical, immobilized chloroplasts and immobilized microbial cells methods have been reported, of the methods, enzymatic method has been usedin the industrial production as the principle of this method is simple and the reactor costs is not expensive. ImcnobiLized microbial cells is also used to regenerate, but the selectivity of the reaction is less than with enzymatic methods and their reactor costs more than the former. Although electrochemical and immobilized chloroplast methods are possible to use for regeneration of NAD(P)H cofactor, they have not been reported in the practical application as yet.
出处
《河南科技学院学报(自然科学版)》
1989年第2期13-19,共7页
Journal of Henan Institute of Science and Technology(Natural Science Edition)