炭粒凝集试验诊断肝吸虫病的初步研究

PRELIMINARY STUDY ON THE DIAGNOSIS OF CLONORCHIASIS BY CHARCOAL GRANULE AGGLUTINATION TEST

本文介绍了用炭粒凝集试验诊断肝吸虫病的初步研究结果。肝吸虫成虫经脱脂、超声粉碎、冷浸处理后致敏活性炭,制成肝吸虫炭粒抗原。对抗原制备及试验操作过程中的有关因素进行了探索,以确定最适宜试验条件。用上述抗原检测肝吸虫阳性血清79份,阳性率83.5％;正常人血清69份,假阳性率2.9％;其它寄生虫病人血清41份,交叉反应率195％。结果表明,本试验具有一定的敏感性、特异性及稳定性,操作简便、快速,不需特殊设备,尤适合基层应用。

This paper reports the results ofdiagnosis of clonorchiasis by charcoal granule agglutination test in a preliminary study. The worms of Clonorchis sinensis were defatted, ultrasonicated and cold-immersed, then the antigens prepared were attached to the charcoal granules keeping in refrigerator ready for test. The factors associated with the preparation of the antigens and the experimentations were explored for determining the optimum experimental conditions. In the detection of 79 positve sera from the patients with clonorchiasis using the antigens mentioned above, the positive rate was 83.5%; while in 69 normal sera the false pcsitive rate was 2.9%; and in 41 sera from the cases infected with other parasites the cross reaction rate was 19.5%. The results show that this method is of certain Sensitivity, Specificity and stability in addition to simple, quick andneeding no sophisticated equipments suitable for application in the grassroots.