用PCMB处理前后人与动物 Hb 电聚焦谱型分析

Analysis of Band Types of Human and Animal Hemoglobin before and after Treatment with PCMB by Isoelectric Focusing

血液(痕)的动物种属鉴别是法医检验工作中的重要内容之一。以往一直沿用免疫学方法。1978年日本大岛美奈子应用聚丙烯酰胺凝胶等电点聚焦(PAG IEF)法,对包括人。

Hemolysates of 41 kinds of animals including human,fetal and monkey's one,were studied by isoelectric focusing on PAG Plate (pH3.5-9.5).Species featuces of Hb band pattern,ability for identification and alternation of Hb band pattern after treatment with P-Chloromercuribenzonate (PCMB) as well as its influence on the species identification of Hb were disscused as well.Approaching to the application of IEF for identifying species gave the following results:Class of animals can be identified hy characteristic distri- bution of the band pattern;Human Hb may be distinguished from that of other animals but monkey's one of which band types somewhat difficultly discriminated because of similar band type,Differences of band patterns are also shown between animals,which may be used for identification.Aftry treatment with PCMB,both human and monkey's Hb are broken down to and β subunit.The different PI value of α subunitin both human and monkey's Hb can be used for identification and much easily to distinguish human Hb from that of the other animals.