应用淀粉/琼脂糖混合凝胶电泳同步检测红细胞EsD和GLOI表型

Detection of Erythrocyte Isoenzymes EsD and GLOI Phenotypes Using a Mixed Starch Agarose Gel Electrophoresis

1973年 Hopkinson 等采用淀粉凝胶电泳发现 EsD(Esterase,酯酶 D)有三种表型即1—1;2—1;2—2型。1975年 K(?)mpf 等也发现了 GLOI(Glyoxala-se Ⅰ,乙二醛酶Ⅰ)同样具有1—1;2—1;2—2三种表型。由于它们都具有遗传多态性,作为遗传标记,受到人类学家、

This paper shows the detection of the phenotypes of isoenzymes EsD and GLOI for fresh blood cells using the same mixed starch agarose gel electrophoresis and Tris-maleic acid buffer system at pH7.4.Among 302 fresh blood samples,the EsD gene frequencies were:EsD^1=0.641,EsD^2= 0.359;Among 401 fresh blood samples,the GLOI gene frequencies:were: GLOI^1=0.1895,GLOI^2=0.8105,It is useful to paternity,test in Nangjing area.