红细胞酸性磷酸酶聚丙烯酰胺等电聚焦电泳分型研究及其在上海地区的分布频率

Study on the Typing of EAP with PAGIF and Distributon of EAP in Shanghai

红细胞酸性磷酸酶(EAP)已被广泛地应用于法医学个人识别中,它具有六种不同的遗传表现型:A、BA、B、CA、CB、C,受控于2号染色体上的三个共显性等位基因Pa、Pb、Pc、中国人群中至今尚未见有 C、CA 和 CB 型的报道。

A method was described for determining erythrocyte acid phosphatase (EAP) phenotypes from fresh human blood and bloodstain by thin-layer polyacrylamide gel isoelectric focusing (PAGIF),obtaining arrow,even, clear isoenzyme bands Some of the advantages of thin-layer PAGIF were increased resulation and dried bloodstain for twomonthes can be detected. Using this method,We detected the EAP phenotypes from 283 unrelated individuals in Shanghai.The results were as follows:EAP A 4.24%,EAP BA 28.98%,EAP B 66.78%;p^a=0.187,p^b=0.813;Did not find p^c.The discriminating power (DP)of EAP was 0.47.