免疫干扰素对急性白血病患者外周血淋巴细胞内环核苷酸水平的影响

EFFECTS OF IMMUNE INTERFERON ON CYCLIC AMP AND CYCLIC GMP LEVELS OF PERIPHERAL BLOOD LYMPHOCYTES OF PATIENTS WITH ACUTE LEUKA'MIA

利用放射免疫分析法研究了急性白血病患者外周血淋巴细胞内环核苷酸(cAMP、cGMP)含量的变化及免疫干扰素对淋巴细胞内cAMP、cGMP水平的影响。结果表明,急性白血病病人淋巴细胞内cAMP含量低于正常人,cGMP含量则升高。免疫干扰素能纠正白血病患者淋巴细胞内环核苷酸水平的异常。

Cyclic AMP (cAMP) and cyclic GMP (cGMP) levels of peripheral blood lymphocytes (PBLS) of patients with acute leukaemia were estimated and compared with those of normal controls in vitro. The results showed that the amount of cGMP of PBLs of cases was higher than that of controls, on the other hand, cAMP level of PBLs of patients and the ratio of cAMP/cGMP were lower than those of normals. However, when PBLs of patients were exposed to interferon-r(200IU) in vitro for 4hr, the content of cGMP was decreased to some extent, and that of cAMP and the ratio of cAMP/cGMP were increasen to normals. The relationships between cyclic nucleotides levels of lymphocytes and cell proliferation, immune function, and the effects of interferon on cyclic nucleotides and cell proliferation, and the possible mechanisms of these effects were discussed.