牛、羊胆囊内肠道菌的分离鉴定

The Isolation and Id entification of Bacteria in Gallbladders of sheep and Cattle

本文对育肥屠宰的牛、羊胆囊进行了细菌学调查,其胆囊内细菌的污染情况是:被检的79例牛羊胆囊,有31例带菌,带菌率为31/79。在32例牛胆囊,有12例带菌,带菌率为12/32;47例羊胆囊,有19例带菌,带菌率为19/47。共分离出菌37株(牛2例、羊4例同时检出2株菌,其余均为1株)。按照Bergey氏《细菌鉴定手册》第8版的分类系统,经鉴定这些菌分别属5科8属中的10种菌(内含1株酵母菌及1株未定菌):大肠埃希氏菌、肠球菌、产气肠杆菌、普通变形杆菌、柠檬酸细菌,粪产硷杆菌、乳酸杆菌、表皮葡萄球菌。调查结果表明:正常情况下牛、羊胆囊内是无菌的。胆囊内检出的细菌是属污染菌,且均为肠道菌。

The article deals with these bacteria remained in gallbladders of fattened sheep and cattle. We have checked 79 gallbladders of which 31 cases are with bacteria; the infected rate accounts for 31/79. of 32 gallbladders of the cattle 12 cases are with bacteria and the infected rate accounts for 12/32; and of 47 gallbladders of the sheep 19 cases are with bacteria and the infected rate accounts for 19/47. These bacteria belong to ten species out of eight genus in five families (including a strain of yeasts and a unknown strain): they are E.Coli, Enterococcus, E.aerogenes, P. unlgaris, Citrobacter, A. faecalis, Lactobacillus, S. epidermidis. Out of 12 samples of gallbladders, seven cases of them have not any change of histology, five cases among the seven no bacteria, but two cases among the seven do have bacteria, five cases of the twelve with inflammation in gallbladders have bacteria. This shows clearly that the main case of cholocystitis comes of bacteria in gallbladders.