大肠杆菌血凝反应的研究

A study on Hemagglutination reaction of Escherichia coli

本文报告不同来源大肠杆菌对不同红血球的血凝反应。使用Evans法,磷酸盐缓冲液琼脂在4℃环境中操作可获满意的结果。人粪源大肠杆菌与豚鼠红血球的MRHA为27.3％,而尿源菌株仅2.7％。人和猪粪源大肠杆菌对A型红血球的MRHA为0～4％,而人尿源株为41.3％。尿源菌株对P2K血球MRHA为41.3％,对血球为12％。尿源菌株对A型与P2K型红血球的MRHA相符率达97％,扫描电镜显示具有菌毛的细菌粘附于A型红血球表面,A型红血球可取代罕有的P2K血球作血凝试验以诊断尿道致病性大肠杆菌。

HA reaction of Escherichia coli from different sources to some kinds of RBC was reported by this paper. Satosfactory results were obtained performing in 4 C condition with Evans methods and phosphatebuffered agar. MRHA of E.coli from faeces of human to RBC of guinea pig occupied 27.3% and 2.7% for E.coli from urine. MRHA of E.coli from faeces of human and pig to RBC of A type occupied 0-4% and 41.3% for E.coli from urine. MRHA of E.coli from urind to RBC of P_2~K type were 41.3% and 12% to type. Corresponding rate of MRHA of E. coli from urine to A type and to P_2~K type was 97%. Fimbriae of bacteria adhesed to surface of A type RBC were showed by scanning electron microscope. A type of RBC can be in- stead of rare P_2~K to identify uropathogenic Escherichia Coli using HA.