用酶联免疫吸附术观察向附睾注射鱼肝油酸钠后抗精子抗体的变化

OBSERVATION ON ANTISPERM ANTIBODY IN SERUM AND SEMINAL PLASMA BY ENZYME—LINKED IMMUNOSORBENT ASSAY AFTER INJECTION OF SODIUM MORRHUATE

本研究用酶联免疫吸附术观察8只家兔在附睾注射鱼肝油酸钠后血清及精浆中的抗精子抗体。血清抗精子抗体阳性率为37.5～50.0%,最早检出率为术后2个月,6～7月达高峰。精浆抗精子抗体阳性率为25%,最早检出率为术后4个月,且此时己达高峰。与输精管结扎资料相比,发现血清抗精子抗体检出率偏低,而精浆相应抗体偏高,表明向附睾注射鱼肝油酸钠引起的免疫反应有别于结扎,以生殖道局部为主。

The serum and seminal plasma antisperm antibody (ASA) was examined by the Enzyme-Linked Immunosorbent Assay(ELISA).In 8 rabbits which had received epididymal injection of sodium morrhuate(SM),Positive rate of ASA was 37.5%-50.0% in the serum.ASA could be detected 2 months after injection and the peak value was obtained at the 6th-7th.Positive rate of ASA was 25% in th seminal plasma,Where it could be detected 4 monthes after injection and when it was detectable,the peak value was obtained.Compared with vasectomy,the positive rate of ASA in the serum after injection of SM was lower,but in the seminal plasma it was higher.It is suggested that the immune reaction followed SM injection may be confined locally in the reproductive tract.