氯化铵对小鼠精子顶体蛋白酶、顶体反应和体外受精的影响

EFFECT OF AMMONIUM CHLORIDE ON ACROSIN ACTIVITY,ACROSOME REACTION AND IN-VITRO FERTILIZATION IN MOUSE SPERMATOZOA

本文从顶体蛋白酶、顶体反应和体外受精三个方面研究了获能前就使用不同浓度的氯化铵对小鼠附睾精子的影响,精子经加有1mM、3mM、10mM和50mM氯化铵的孵育液孵育1小时后,用明胶底物膜法测定顶体蛋白酶活性,孵育2小时后制作电镜标本,观察顶体反应的发生情况,并用体外受精的方法检测精子的受精能力。结果,NH_4Cl在高浓度(≥10mM)时,能抑制小鼠精子的顶体蛋白酶活性和顶体反应,且药物浓度越高,抑制越明显,当NH_4Cl浓度为50mM时才对精子的体外受精能力产生抑制作用。提示从抑制溶酶体内容物释放方面寻找抑制顶体反应的药物作为避孕的新途径是切实可行的。

This study describes the effect of NH_4Cl on acrosin activity,acrosome reactions and the fertilizing ability of mouse epididymal spermatozoa.Ammonium Chloride was added to the incubation media of spermatozoa with final concentrations of 1,3,10 and 50mM.After one hour incubation,acrosin activity was tosted by using fixed gelatin membrane method.After two hours incubation,the aorosome reaction was quantitated under electron microscope.The fertilizing ability of sperm was tested by in-vitro fertilization test.The results showed that NH_4Cl could obviously inhibit the acrosin activity and acrosome reaction of spermatozoa at higher concentrations≥10mM at a dosedependent manner.The authors suggest that one could search for contraceptives out of those drugs which inhibit the release of lysosomal constituents.