斑点酶联免疫吸附试验检测血清HBsAg的初步研究

A Preliminary Investigation on the Detection of Hepatitis B Surface Antigen in Sera by Dot Immunoassay

用斑点酶联免疫吸附试验(Dct Immuno assay DIA)检测146例血清中乙肝病毒表面抗原(HBsAg)。结果表明DIA和反向被动血凝(RPHA)的阳性率分别为34.9％和30.8％,二者无显著差异(P>0.05)。它和RPHA一样简易快速,但DIA所用血清量极少(5μl),纤维膜上的阳性结果可以长期保存。试验结果稳定,用48份样品重复三次,阳性结果没有变化。DIA检出的HBsAg滴度比RPHA约高4～10倍。

Hepatitis B surface antigen (HBsAg) in 146 sera specimens were detected by dot inmmunoassay (DIA). The results showed that the positive rates by DIA and by RPHA were 14.9% and 30.8% re spectively. There were no significant differences between the two (P>0.05).It is as simple and rapid as RPHA, but the volume of specimen needed by DIA was more little (5 ul). The positive results on the sheet could be preserved for a long period of time. The stability of the test is good, the test has been reproducted three times. Using 48 specimens, the results were no change, the titers of DIA were 4—10 times higher than that of RPHA