从我国肠道传播的非甲非乙型肝炎病人粪便中分离出一株小RNA病毒

ISOLATION OF A SMALL RNA VIRUS FROM FECES OF A PATIENT WITH ENTERICALLY TRANSMITTED NON-A NON-B HEPATITIS IN CHINA

从我国新疆肠道传播的非甲非乙型肝炎(NANBH)患者的急性期粪便中,用细胞培养分离到一株致细胞病变(CPE)和有血凝活性的病毒.分离的病毒能被新疆NANBH病人急性期混合血清所中和,在血凝抑制试验中病毒血凝能被病人血清所抑制,在免疫电镜检测中可与1∶160稀释的新疆NANBH病人血清起凝聚作用,而与甲型肝炎病人急性期混合血清仅在中和试验和免疫电镜中存在低滴度交叉现象,在血凝抑制试验中无交叉反应.在电镜下观察细胞培养物可见到直径为30～34nm圆形病毒颗粒,与病人粪便中见到的病毒颗粒相似.理化试验结果显示分离的病毒属无包膜的、耐酸的RNA病毒,在56℃水浴中作用30min,感染力降低.应用微量中和试验和血凝抑制试验测定5例患者双份血清的结果表明,有2例抗体有4倍或4倍以上升高.我国用细胞培养方法分离出肠道传播的NANB H病原体获得成功,这对该病毒的分子生物学研究、特异性诊断、流行病学调查以及防治都有重要意义.

A strain of small RNA virus producing cytopathic effect and hemagglutination was isolated from fecal sample of a patient with enterically transmitted non-A,non-B hepatitis hy cell culture in Xinjiang,China.The isolated virus could be neutralized by sera of patients,and appeared to be aggregated under IEM,but it had lower titer cross reactions with sera of patients with HAV and apparently normal persons.Spherical virus particles,30-34nm in diameter,were found in cell cultures under IEM,the characteristics of the virus particle were found to be similar to those in the fecal sample of the patient.The results of physico-chemical studies revealed that this RNA virus is acid-fast,ether-resistent and heat-sensitive.Micro-neutralization and hemagglutination inhibition tests revealed that 2 of 5 paired patient sera showed 4-fold or more increase in antibody titer.We for the first time successfully isolated this small RNA virus that is very important for the further study on the diagnosis,prevention and treatment of non-A,non-B hepatitis.