金黄色葡萄球菌青霉素酶的简易检测方法

SIMPLE METHOD FOR DETECTION OF PENICLUNASE OF STAPHYLOCOCCUS AUREUS

本文用简易青霉素酶测定法——纸片法检测1005株金黄色葡萄球菌(金葡菌)产昔霉素酶的能力,与液体碘测定法和产色头孢菌素法比较,显示该法与产色头孢菌素法具有同样简便、快速、灵敏、准确的特点。液体碘法中有5株结果与上述二法不符,三种方法的符合率为99.5％。金葡菌产青霉素酶的能力与其对青霉素G的敏感性有密切的相关性,不产酶株均敏感。青霉素酶检测纸片法可代替常规药敏纸片法测定金葡菌对青霉素的敏感性,凡此法示不产酶者,可以青霉素为首选药物。

Qualitative detection of penioillinase in 1005 clinical isolates of Staphylococcus (Staph) aureus was carried out by the penioillinase disc method. It was compared with the conventional liquid iodometric and the ohromogenio oephalosporin(oefinaae disc) methods. The penioillinase disc method is simple, sensitive, rapid and accurate. The results were identical to those of the oefinase disc method. Only five strains showed false positive reactions by the liquid iodometrio method, proved by the penicillin G disc mebhod. The correlation rate for these, three methods was 99.5%. Non-penioil-linase producing Staph aureus were sensitive to penicillin G. The minimum inhibitory concentration (MIC) of penicillin Q was significantly different between the strains with and without penioillinase production. However, the susceptibilities-of these two groups of Staph aureus to enzyme-stable methioillin and three eephalo-sporins were also different, although not statistioaly significant. Detection of penieillinase is the qualitative reflection of bacterial .resistance. It is suggested thai the penioillinase disc method can be employed in clinical bacterial laboratories to replace Staph aureus penicillin G disc susceptibility testing. Penicillin G is still the antibiotic of choice for Staph aureus infoetion, which, penicillinase is not produced or detected by the penicillinase disc method.