细胞内分裂及细胞内复制诱导

INDUCING OF ENDOMITOSIS AND ENDOREDUPLICATION OF DON CELLS

以中国雄性仓鼠饰成纤维细胞(Don细胞株)为实验材料,用二乙基己烯雌酚(DES)作诱导物,诱使细胞进行细胞内复制和细胞内分裂。实验采用不同剂量、不同处理时间和恢复时间,取得了良好的结果。在18个试验组中,有50％的试验组获得成功,最高诱导频率达56％,是前人研究结果的2.4倍,同时降低了诱导物质的毒性,加大了有效诱导剂量与毒害剂量间的差距,使有效诱导剂量的变化,高低相差10倍;使发生诱变的细胞大量存活,同时进行分裂活动。

In the research, the Don cells were treated with DES(diethyl stilbestrol) to induce endomitosis and endoreduplication using different dosages of DES and time of treating and recovering.Good result was obtained in the test , that 56.3% of the cells in mitosis was shown as polyploid and endoredupliceating cells in one of the test groups; 36,5% of endoreduplicating cells in another group.The percentage of induced cells was 2,4 times higher than that by Jackson, and it was also found that DES was a good inducing agent to obtain endomitosis and endoreduolication whose toxicity was low to the cells and large in the differences between the effective dosage and poisonous dosage of the chemicals using to induce endomitosis and endoreduplication.In the range of effective dosage the highest was 10 times as high as the lowest.