姜黄素对哮喘小鼠α-SMA的表达及气道重构的影响

Effect of curcumine on expression of α-smooth actin and airway remodeling in asthmatic mice

目的:观察腹腔注射姜黄素对小鼠α-平滑肌肌动蛋白(α-SMA)表达的影响,验证其对哮喘小鼠气道炎症的防治和对肺的保护作用,探讨姜黄素在哮喘气道重构的治疗作用.方法:36只雄性SD小鼠随机分为正常对照组、哮喘模型组、姜黄素治疗组各12只,采用卵蛋白致敏小鼠哮喘模型,ELISA检测支气管肺泡灌洗液中浓度并进行嗜酸粒细胞计数,HE染色观察各组气道炎症发生及气道结构改变情况,同时采用免疫组化法检测α-SMA在肺组织的表达.结果:正常对照组、哮喘模型组、姜黄素治疗组的EOS分别是(0.74±0.26)×108/L,(5.32±0.41)×108/L,(3.55±0.46)×108/L,哮喘模型组明显比正常对照组增高,两组比较有统计学意义(P<0.01).哮喘模型组小鼠具有明显的气道炎症,以淋巴细胞、嗜酸粒细胞为主,出现上皮细胞增生、平滑肌肌层增厚、结缔组织增生、黏液分泌旺盛并伴小气道栓塞.支气管黏膜下、血管壁及周围、肺间质均有大量胶原纤维沉积.姜黄素治疗组小鼠炎症明显改善,黏液产生减少,上皮增生、平滑肌层增厚不明显,气道周围胶原纤维及黏液颗粒均减少,无明显气道痉挛.正常对照组α-SMA的表达阳性率为8%(1/12),哮喘模型组达58%(7/12),姜黄素治疗组为33%(4/12),两两比较差异有统计学意义(P<0.01).结论:姜黄素能够抑制α-SMA的表达,减轻气道炎症反应、延缓气道重构进程.

AIM： To investigate the influence of curcumine on α-smooth actin（SMA） expression and to explore the role of eureumine in asthmatic mice. METHODS： Thirty-six male mice were randomly divided into normal control, experimented group and curcumine treatment group, 12 mice in each group. The mice were sensitized and challenged with ovalbumin to establish the asthmatic model. Bronchoalveolar lavage fluid was collected and the number of white blood cell and eosinophilic granulocyte （EOS） was counted. Airway inflammation and alteration of airway structure were detected by HE staining and α-SMA expression in pulmonary tissues was detected by immunohistochemistry. RESULTS： The counts of EOS in normal control, experimented group and eurcumine treatment group were （0.74 ± 0.26）, （5.32 ± 0.41 ） and （3.55 ± 0.46 ） × 10^8/L respectively. Experimented group was significantly higher than normal control group （P 〈0.01 ）. Airway inflammation was extensive in experimented group. Prominent proliferation of airway epithelial cells, thickness of smooth muscle, hyperplasia of connective tissue, and a marked increase in airway mucus secretion with mucus plugging were observed in experimented group. A great deal of collagen deposition was found in the bronchus submueosa, the afferent wall and surroundings in experiment group. The inflammation was significantly decreased in curcumine treatment group, with decreased production of mucus, decreased collagen and granule of mucus aroundairway, and less proliferation of airway epithelium, smooth muscle hypertrophy and airway spasm. The expression of α-SMA in control group, sensitized group and asthma group were 8.34% （1/12）, 58. 33% （7/12） and 33. 33% （4/12）respectively. There was statistical significance between the groups（P 〈0.01 ）. CONCLUSION： Curcumine inhibits the expression of α-SMA, thus relieving airway inflammation and delaying the process of airwayremodeling.