西瓜杂种及其亲本同工酶分析

ISOZYME ANALYSIS OF HYBRIDS AND THEIR PARENTS ON WATERMELON [Citrullus lanatus (Thanb.)Mansf.]

以西瓜的10个杂种及其16个亲本为试材分析了干种子、发芽期、子叶期的根及子叶的过氧化物酶(POD)同工酶和酯酶(EST)同工酶。结果表明西瓜POD同工酶和EST同工酶酶谱在不同时期,不同部位不尽相同。发芽期及子叶期POD同工酶和子叶期EST同工酶存在着杂种与亲本差异,杂种酶谱表现为父本相同、母本相同、双亲相同、出现“杂种酶”带及缺少双亲某些酶带五种类型。其中POD同工酶与其父本相同和EST同工酶与其母本或父本相同的杂种多表现较强的杂种优势。

10 F_1 hybrids and their 16 parents of watermelon [Citrullus lanatus(Thanb.)Mansf.] were used for isozyme analysis of peroxidase(POD)and esterase(EST) of the dry seeds, gerrninating seeds, cotyledons and roots at cotyledon stage. The results obtained indicated that zymograms of POD and EST varied at different stages and different parts of the plant. Isozyme variation between hybrids and their parents was found at seed germination stage and cotyledon stage of POD, as well as cotyledon stage of EST. Following cases were observed in our study: F_1 hybrids had the same zymogram as their two parents, or maternarl parents, or paternal parents, or had 'hybrid enzyme' or to be lack of some zymographic bands of their parents. High heterosis was generally found in the hybrids with same isozyme zymograms of POD as their paternal parents, and EST as their maternal parents.