大鼠失血性休克时中枢两种脑啡肽含量变化及其发病学意义

The Changes of the Enkephalins' Contents in the Brain Areas of Rats During Hemorrhagic Shock and Their Pathogeentic Significance

本文用放射免疫测定法观察大鼠失血性休克时中枢甲硫氨酸脑啡肽(MEK)和亮氨酸脑啡肽(LEK)含量的变化,并观察侧脑室注入纳洛酮和脑啡肽抗血清对休克的影响。结果表明延髓MEK和垂体LEK在休克60分钟即明显降低,210和360分钟时进一步下降;纳洛酮预处理可部分防止休克早期的心率减慢,并可在恒定贮血瓶压力下增加相同时间内的失血量;LEK抗血清(滴度1:20000)可部分防止休克早期心率减慢,而对失血量无影响,MEK抗血清(滴度1:5000)对失血性休克未见明显影响。

In order to evaluate the role of enkephalins in the pathogensis of hemorrhagic shock, we measured MEK and LEK in 4 brain areas of rats during the shock by RIA, and observed the effects of intravemricular preventive treatment with naloxone or antisera of MEK and LEK on the shock. The results showed that: (1) The levels of MEK in medulla oblongata and LEK in pituitary decreased significantly at GO min. Of hemorrhagic shock of rats, and decreased further at 210 min. and 350 min. (2) Intraventricularly preventive treatment with 100 |ig of naloxone could partly prevent the brt-dycardia in the early stage of shock and simultaneously increase the volume of shed blood (3) Antiserum of LEK (with the titer of 1:20000) could partly prevent the bradycardia, but had no effect on the volume of shed blood. (4) Antiserum of MEK (1:5000) had neither effect on heart rate nor on shed blood volume. The pathogenetic significance of the above-mentioned changes is discussed.