小儿糜烂性口腔炎病毒病因及干扰素对病毒的抑制作用

Isolation of Type 1 Herpes Simplex virus from Childhood Erosive Stomatitis and Inhibitory Effect of Leukocyte Interferon on the Virus

本文研究了小儿糜烂性口腔炎的病毒病因,用人胚肾细胞及Hep—2细胞分离病毒,26例患儿有21例阳性(80.7％),从中取五株病毒进行鉴定,细胞病变特点属疱疹病毒,且均可被抗HSV—I抗体中和,结果表明HSV—I是本病的主要病原。体外实验证明,白细胞干扰素对新分离的HSV—I有较强的抑制病变作用,2.4～2.9Iu/ml白细胞干扰素即可抑制病毒的50％CPE。临床上试用干扰素局部治疗必获得显著的疗效。

Viral etiological study of childhood erosive stomatitis was done. Twenty one strains (80.8%)of viruses were isolated from 26 patients by inoculating cultured Hep-2 cells and human embryonic kidney cells with materials collected from the macerative and vesicular lesions of the mouths. Five strains of the virus chosen at random were identified as Herpes Simplex Type 1 by their characteristic cytopathic changes in the infected tissue culture cells and by neutralization text with anti-adenovirus Type 3 and Type 7 immune serums. In vitro study showed that human leukocyte interferon could effectively inhibited the cytopathic effect induced by the newly isolated viruses, the 50% inhibiting doses being 2.6-2.9 I.U. per milliliter of the interferon. Signficant therapeutic effect was obtained by local application of the interferon for treating 50 children with macerative stomatitis.