各类型麻风皮损内T细胞亚群的研究

In Situ Characterization of T Lymphocyte Subsets in Various Types of Leprosy.

本文应用单克隆抗体和免疫酶标技术,分析了30例各类型麻风皮损(包括TT6例、BT4例、BB1例,BL6例及LL13例)内Leu4、OKT4、及OKT8细胞的数量及其分布特点,发现Leu4细胞在TT数量较多,并多分布在肉芽肿周围,而在LL则量少而(氵弥)漫分散于病灶内。OKT 4/OKT8平均比值从TT端移行至LL时呈逐渐下降趋势。而LL的OKT8细胞相对增多。TT及BT尚见OKT4与OKT8细胞分布上呈分离现象。这些均说明TT及BT病变免疫反应较明显。本文尚发现表皮与毛囊内有OKT 4及OKT 8细胞浸润、较多见于TT及BT、但OKT 8细胞浸润较多见于LL。

Using monoclonal antibodies and immunoperoxidase techcique, the num bers and distribution of T lymphocytes subsets in the skin lesions of various types of leprosy (including TT 6 cases, BT 4 cases, BB 1 case, BL 6 cases, and LL 13 cases) were determined and compared with each other. We found large amount of Leu 4 cells at the periphery of the TT granuloma, while the least number of this cells scattered loosely and admixed with the leprous cells in LL. The OKT4/OKT8 ratio was much higher in the TT polar type, with gradually declining towards another pole of the spectrum in LL type, c. g., TT(1.76)>BT(1.1)>BL(1.03)>LL(0.45). It showed that OKT8 cells were relatively increased in LL. The pattern of segregation of OKT4 and OKT8 cells at the periphery of the granuloma were shown in TT as well as in BT, but was better developed in the former. All these may reflect immunohistolcjgically a cell-mediated immune response seen in both TT and BT. In addition, we found infiltration of OKT4 and OKT8 cells in epidermis as well as in the hair follicles, which were easily seen in TT and BT. Infiltration of OKTg cells were encountered in LL, and their signifincance was also discussed.