生物素标记HBV-DNA探针的临床应用

Clinical Application of Biotinlabled HBV-DNA Probe

本文报告用自制的生物素HBV-DNA探针进行斑点杂交检测HBV感染患者血清中的HBV-DNA,并与同位素探针相比较。用生物素探针测定HBV-DNA之阳性率在HBsAg和HBeAg同时阳性者中为84.2%,HBsAg和抗-HBe同时阳性者中为23.5％,单纯HBsAg阳性者中为18.6％结果表明生物素HBV-DNA探针其特异性、敏感性和重复性与同位素探针相似,且生物素探针有效时间长,稳定性好。

A method for the detection of HBV-DNA in serum by spot hybridization using bio-tinlabled HBV-DNA probe is reported. The serum samples of 317 patients with HBV infection wer; tested for HBV-DNA by biotinlabled probe. It was found that the positive rates of serum HBV-DNA were 34.2%, 23.5% and 18.6% in both HBsAg and HBeAg positive, both HBsAg and anti-HBe positive and single HBsAg positive patients, respectively. The results indicate that biotinlabled HBV-DNA probe posseses similar spicificity, sensitivity and repetitivity in comparison with radio-active isotope labled probe. However, the stability of biotinlabled probe is better than the letter's.