猪卵透明带单克隆抗体对人卵巢免疫组织化学研究

THE IMMUNOHISTOCHEMICAL STUDY OF HUMAN OVARIN TISSUE USING MONOCLONAL ANTIBODIES AGAINST PORCINE ZONA PELLUCIDA(ZP-OVA)

从猪卵巢中分离卵子提取透明带(ZP—OVA).实验研究证明对卵巢功能有一定影响.我们制备的三株抗猪ZP—OVA单克隆抗体(LPD_8,LPC)4.LPD_2,,其中二株(LPD_8,LPC_4)以免疫组织化学方法对人卵巢组织进行体外试验.发现它们除对人卵透明带有交叉反应外,还在卵细胞质内见有反应颗粒,而对卵泡颗粒层及卵泡膜则无反应。这说明卵细胞质内有与上述单抗结合的抗原决定簇,因此证明透明带与卵细胞质有共同抗原成分,这一现象支持透明带(ZP—OVA)

Three hybridomas producing monoclonal antibodies (Mab) to zona pellucida of porcine ovarian egg(ZP-OVA) were established(LPDg, LPC4, LPD,).Two Mab(LPD_8 LPC_4) were examined against human ovarian tissue by immunohistochemical technique in vitro. We found that the two Mab had cross-reaction with zona pellucida and ovum cytoplasm and no cross-reaction with membrana granulosa and theca folliculi. It showed that there were affinities of LPD_8 and LPC_4 to antigenic determinamts in the ovum cytoplasm and common antigenic components in zona pellucida and ovum cytoplasm. It suggested that the zona pellucida was derived from oocyte.